Ant far from building

December 28, 2025

Sample information
Picture
Entry by: Ronan K.
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Collected from an exposed ant colony on eroded bluff far from the school building.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica Formica glacialis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes
  • Sample initially preserved in ethanol, then rinsed before dissection.
  • Buffer ATL, AL, and Proteinase K were used to lyse cells and isolate DNA.
  • DNA then purified with buffers AW1, AW2 and centrifuging with a spin column.
  • DNA then eluted with Buffer AE

Extraction as described above went as planned.

Wolbachia PCR in lane 5 of image

Arthropod PCR in lane 4 of image

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Tested positive, but everyone in the class had positive test, so possible contamination of samples.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Formica glacialis was found to be postive for Wolbachia.
Report Inappropriate Post