Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | The ant was collected right outside the Drake hockey arena from an antpile in the grass. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Formica Formica glacialis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I had a Wolbachia sequence and an arthropod sequence. There were also bands in the gel electrophoresis for Wolbachia and arthropod DNA. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Formica glacialis was found to be postive for Wolbachia. |
European Paper Wasp
Woodworm Ant