Mosquito-Piper Floyd

September 15, 2026

Sample information
Picture
Entry by: Piper F
Location
Collection date 09/06/2025
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

I found this at night on my front porch under a light.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am worried there was human error during the DNA extraction process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post