Sample information
Picture	Photos by: Aniyah J.
Location
Collection date	10/15/2025
Captive / Cultivated?	Wild-caught
Group	Oak Ridge High School
Observations
Putative identification	Arthropoda Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SeeGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Steatoda triangulosa was found to be negative for Wolbachia.