Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Oak Ridge High School |
| Observations | |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Solenopsis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls work and through research there was no presence of Wolbachia in fire ants in north America but is present in South American fire ants where they are native to |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Solenopsis was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica