MAC-NTM Coleoptera

November 10, 2025

Sample information
Picture
Photos by: Nisa M.
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Red and blue beetle found in tall grass
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m very confident in my results since my controls were correct and there was a 22.5% chance of Wolbachia in my insect.
Wolbachia 16S sequence
Arthropod COI sequence
GgTtTAGTAGGAACTTCTTTAAGAATATTAATTCGAACAGAGCTAGGTAG CCCCgtaTCATTAATTGGAAATGACCAAATCTATAATGTAATTGTTACAG CCCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTACCACTAATAATTAGGGCCCCTGATATAGC ATTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCTTCACTAT TTTTACTAATTATAAGAAGAATTGTTGAAAATGGAGCAGGAACAGGATGA ACTGTATATCCCCCTTTATCATCTAACTTAGCTCATGAAGGATCTTCAAT TGATTTAGCAATTTTTAGACTACATTTAGCAGGAATTTCTTCTATTCTTG GAGCTATTAATTTTATTACAACAGTAATTAATATACGACCAATTGGTATA ACCCTAGATCAAATACCATTATTTGTATGGGCTGTAACAATTACAGCTAT TTTATTACTATTATCTTTACCAGTATTAGCAGGAGCTATCACAATATTAT TAACTGATCGAAATTTAAATACTTCTTTTTTTGACCCTGCAGGAGGAGGT GATCCTATCCTTTATCAACACCTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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