Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/25/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | My confidence level is high because when I measured my specimen’s DNA it was good quality, 2.2. I also took my time in doing everything and made sure to double check that I was doing all the activities right. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TCTCTTTGGtttaTGAGCTGGGaCTGTAGGCACTTCTTTAAGTTTTTTAA TTCGAGTTGAGTTAGGAAGTCCTGGGCAAGTGTTAGGTAATGATCAGATT TATAATTCTGTTGTTACGGCTCATGCTTTTATTATAATTTTTTTTTTTGT TATACCATTTATAATTGGGGGGTTTGGTAATTGATTAGTTCCTTTAATAA TATCTATTCCTGACATaGCTTTTCCTCGTATAAATAATATGAGTTTTTGA TTATTACCACCTTCATTATTATTTTTAACTATAGGAATATTTATTGGGGA TGGTGCAGGGACTGGTTGAACTGTATATCCACCTCTTTCTAATTCAATTA ATCAATCAGGACCTagaGTagaTTTATCAATTTTTTCTTTACATATTGCT GGGGCATCATCTATTATAGGGGCAATAAATTTTATTATTACAATCTTTTT GTCAGTAcgaACTTCTATAGATAAAATTTTTTTATTTTGTTGATCTGTAG TAATTACAGCAATTTTATTATTATTATCTTTACCTGTTTTAGCGGGAGCA ATTACTATGTTATTATTTGATCGAAATTTTAATACTACTTTTTTCCTTCC TTTAGGGGGTgGaGATCCTGTTTTATATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid