JOE-JAS Diptera

November 10, 2025

Sample information
Picture
Photos by: Justin S.
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insects collected near Georgia Southern University Armstrong campus, nearby Science Center parking lot. Insect collected via beating net from shrubs. Collected at approximately 6-7 pm EST.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No band consistent with the base pairs of Wolbachia fluoresced when gel was exposed to UV light.
Wolbachia 16S sequence
Arthropod COI sequence
TtgGaACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATCCAGG ATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
Report Inappropriate Post