Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/08/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Long legs, has wings, red and black colors |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The DNA showed up very clear on the gels |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGGTTTAGTAGGAACTTCTTTAAGATATTAATTCGAACAGAGCTAGGTAG CcccgttatggtaATTGGAAATGACCAAATCTATAATGTAATTGTTacct ttcATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTACCACTAATAATTAGGGCCCCTGATATAGC ATTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCTTCACTAT TTTTACTAATTATAAGAAGAATTGTTGAAAATGGAGCAGGAACAGGATGA ACTGTATACCCCCCTTTATCATCTAACTTAGCTCATGAAGGATCTTCAAT TGATTTAGCAATTTTTAGACTACATTTAGCAGGAATTTCTTCTATTCTTG GAGCTATTAATTTTATTACAACAGTAATTAATATACGACCAATTGGTATA ACCCTAGATCAAATACCATTATTTGTATGGGCTGTAACAATTACAGCTAT TTTATTACTATTATCTTTACCAGTATTAGCAGGAGCTATCACAATATTAT TAACTGACCGAAATTTAAATACTTCTTTTTTTGACCCTGCAGGAGGAGGT GATCCTATCCTTTATCAACACCTATTTTGATTTTTTGGTCACCCTGGAAG T
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid