Wolbachia(+) Formica argentea

November 10, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 06/22/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Collected live in hotel parking lot.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica Formica argentea

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results are third row. Top: CO1. Bottom: Wol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Tests worked fine.
Wolbachia 16S sequence
TTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGCTCATCAAATTCACTAATCAATAACGATCAAATTTATAATTCTTTAGTAACTAATCATGCTTTTATCATAATTTTCTTCATAGTAATACCATTTATAATTGGAGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCTCCAGACATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCCTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGCACAGGTACAGGATGAACTATTTATCCCCCTTTAGCTTCAAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTACATATTGCAGGTATATCATCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTTGTATGATCAATTTTAATCACAGCTATCTTACTTCTATTATCCTTACCTGTTTTAGCCGGAGCTATTACTATACTATTAACTGACCGAAATTTAAATACCTCATTTTTTGACCCTTCAGGAGGGGGCGACCCAATTCTCTATCAACATTTATTTTGAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica argentea was found to be postive for Wolbachia.
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