Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 06/21/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Collected live near a light fixture. |
| Putative identification | Arthropoda Insecta Ephemeroptera Isonychiidae Isonychia Isonychia rufa |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Results are fifth row. Top: CO1. Bottom: Wol. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Tests worked fine. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGGCAGGAATAGTAGGAACTTCCCTTAGTCTTTTAATTCGAGCTGAGTTGGGACAACCAGGATCATTAATTGGTGACGATCAAATTTATAATGTTATTGTTACAGCTCATGCTTTCGTTATAATTTTCTTCATGGTTATACCCATTATAATTGGAGGGTTTGGAAATTGATTGGTACCTCTTATACTTGGAGCTCCCGATATGGCCTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCAGCCTTAACTTTACTCTTGGCCAGCAGTATAGTAGAAAGAGGAGCTGGCACCGGGTGGACTGTTTACCCCCCACTTTCTGCGGGTATTGCCCATGCAGGAGCTTCAGTAGATCTTGCTATCTTTTCTCTTCATTTAGCAGGAGTTTCTTCTATTTTAGGAGCAGTAAATTTTATTACTACTACTATTAACATACGTTCTAGTGGAATAACTATAGATCGTATTCCCCTCTTTGTTTGATCTGTACTTATTACTGCTATTCTCCTCCTTTTATCCCTCCCAGTCCTTGCCGGAGCAATTACCATATTGTTAACAGACCGAAACTTAAATACTTCCTTTTTTGACCCAGCAGGAGGGGGAGATCCTATTTTATATCAACATCTCTTTTGATTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Isonychia rufa was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica