Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 06/23/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Collected live crawling in grass in city park. |
| Putative identification | Arthropoda Insecta Hemiptera Miridae Chlamydatus Chlamydatus wilkinsoni |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Results are sixth row. Top: CO1. Bottom: Wol. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Tests worked fine. BLAST results for CO1 are not a perfect match. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GAATTGAACTAGGTATACCTGGCTCATTTATCGTAGACGATCAAACTTATAATGTAGTAGTAACAGCCCACGCATTCATTATAATTTTCTTCATAGTTATACCAATTATAATTGGTGGGTTTGGAAACTGGCTAATACCNTCAATAATTGGAGCCCCCGATATGGCATTCCCTCCAATAAATAATATAAGATTTTAATTACTCCCCCCCCNTATTACATTATTATTAACTAGAAGAATANTAAAAAATGGAGCAGGAACTGGATGAACATTCTTCCCCCCCTCCCCTNTTNNTTTTGACNTGCGGGGGNCTCGATNTAATTAACATCTTTTTATTTCNCTCTTCNNNGTGTCATANNACCTTNTATCCGAGAATTTTATCTTCTTANTTCTANNATAATAATCAAANGAATTACCGTTTNACCTATTCCNCNNATNCNATTTCTTGGATGAATCTCAACNTCTACTTGATTGCTTTCCCTTTCNTTACCTNCGGNNCNTGNCCGAACCCCTCACAGATTGTAAATATANNNATCNTTCTTCTCTTCCTNTTGCGGNGGNGGANGGNGNNGANANCANACTTATATNACATTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Chlamydatus wilkinsoni was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid