MAT-SKL Hymenoptera

November 12, 2025

Sample information
Picture
Photos by: Skyla L.
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

2 wings, 6 legs, 3 body parts, 2 antennae
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I caught the insect, extracted its DNA, conducted a PCR test, and received results that were proper and as expected.
Wolbachia 16S sequence
TGGCGTGCTGATCCACGATTACTAGCGATTCcatttTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTTTGCATATGATCAggaatAaTTGGATCTTCCTTAAGAATAATTATTC GAATAGAATTAAGAATTCccgtatCATGAATTAGTAATGACCAAATTTAT AATTCCTTTATTACattgcACGCATTTTTAATAATTTTCTTTTTAGTTAT ACCGTTTTTAATTGGTGGATTTGGAAATTGACTCACACCTTTAATATTAG GAGCCCCTGATATAGCTTTCCCCCGTATAAATAATATTAGATTTTGATTA TTACCCCCCTCATTATTAATATTATTATCAAGAAATTTAATTAACCCAAG ACCTGGAACAGGATGAACAGTTTATCCACCACTATCTTCTTATACTTATC ATCCTTCTCCTTCTGTAGATCTAGCAATTTTTTCTTTACACTTATCAGGT ATTTCTTCTATTATTGGATCAATAAATTTTATTGTAACAATTTTATTAAT AAAAAATTATTCAATAAATTATAATCAAATACCTTTATTTCCCTGATCTG TTTTAATTACAACAATTTTATTACTATTATCACTACCTGTATTAGCAGGA GCAATTACAATATTATTATTTGATCGAAATTTAAATTCATCATTTTTCGA CCCTATAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTT TTGGTCACCctgAAGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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