DEM-KTP Odonata

November 12, 2025

Sample information
Picture
Photos by: Kyleen P.
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in grassy field, few shrubs/trees, some dead grass.
Caught using sweep net.
Putative identification Arthropoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls came out as expected. Statistically, insects in the order Odonata tend to have a lower prevalence of Wolbachia infection. Statistics are especially relevant in the region where this experiment was carried out. In this region, Wolbachia infection prevalence in insects in the Order Odonata is 20%, which in the grand scheme of things is not much.
Wolbachia 16S sequence
Arthropod COI sequence
TAGTAGGTACAGCGCTGAGAGTTTTAATTCGGATTGAATTAGGTCAGCCA GGGTCTCTAATTGGTGATGATCAAATTTATAATGTAATTGTAACTGCCCA CGCTTTTGTAATAATTTTTTTTATAGTTATACCTATCATAATTGGAGGTT TTGGTAACTGACTAGTACCTTTAATATTAGGAGCTCCTGATATGGCCTTC CCACGATTGAATAATATAAGTTTTTGATTATTACCTCCTTCCTTTACACT ACTTTTAGCTAGAAGTATGGTAGAAAGTGGTGCAGGAACAGGATGAACAG TTTATCCCCCATTGGCAGGAGCTATTGCCCATGCTGGTGCATCTGTTGAC TTAACCATTTTTTCATTGCATTTAGCTGGTGTGTCATCAATTTTAGGGGC CATTAATTTTATTACAACAGTAATCAATATAAAATCCCCTGGAATAAGTA TTGATCAACTACCATTATTTGTATGGGCAGTAGTAATCACTGCAGTATTA CTTCTTTTATCATTACCAGTACTAGCTGGTGCTATTACCATGCTGTTAAC TGATCGTAATATTAATACATCTTTCTTTGACCCAGCAGGAGGTGGAGACC CAatcTTATACCAACACTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Odonata was found to be negative for Wolbachia.
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