Ladybug (Coccinellidae septempunctata)

November 14, 2025

Sample information
Picture
Entry by: Himanshi K
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

  • The insect is very active.

  • It was actively trying to climb out of the container.

  • Its wing covers are bright red.

  • It has small black spots on its back.

  • It appears to be a medium-sized ladybug.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Coccinella Coccinella septempunctata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

We used precise measurements using a micropipette and other lab tools.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The clear bands from our gel electropheresis and the quality score above 40 indicate that the ladybug was Wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGANATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTTATTTTGATTTTTTGNCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coccinella septempunctata was found to be negative for Wolbachia.
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