Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | long, spindly legs; positive human interaction; able to climb; 8-legs; mandibles; brown body |
| Putative identification | Arthropoda Arachnida Opiliones Sclerosomatidae Leiobunum Leiobunum vittatum |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | We are not very confident in our results as none of our samples showed bands for Wolbachia and we are concerned about potential human error during the DNA extraction and PCR process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Leiobunum vittatum was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR