Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | brown/black; 8 legs; large mandibles; fast-moving; good climber |
| Putative identification | Arthropoda Arachnida Araneae Gnaphosidae Scotophaeus Scotophaeus blackwalli |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | We experienced no bands on our gel for Wolbachia and are unsure if the results are accurate because of potential human error somewhere in the DNA extraction and PCR process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Scotophaeus blackwalli was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR