Millipede (Ophyiulus pilosus)

November 13, 2025

Sample information
Picture
Entry by: Himanshi K.
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

  • It was found beneath the soil.

  • It demonstrated low-resistance behavior when handled.

  • The body is brown.

  • It has ringed patterning.

  • The body is thin and elongated.
Putative identification Arthropoda Diplopoda Julida Julidae Ophyiulus Ophyiulus pilosus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

We used precise measurements using a micropipette and other lab tools.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We did not send our Wolbachia for sequencing, so we are confident in our results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ophyiulus pilosus was found to be negative for Wolbachia.
Report Inappropriate Post