Brown Marmorated Stink Bug

November 13, 2025

Sample information
Picture
Entry by: Tanashri G.
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations
  • Active
  • Jumped into container
  • Walks a lot
  • 6 legs- 3 on each side
  • Brown with a beige pattern
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Our group worked productively with our time and used precise measures when working with all the samples and parts of the experiment.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The stinkbug was not sent for Wolbachia screening, hence the confidence level stays high.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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