Sample information

It was found under a moist rock around 7am still alive, it was around 40 degrees fahrenheit outside. And when placed in the freezer its legs curled up.

Methods

DNA extraction : I made sure to take the legs off the spider and only crush the abdomen of both the spider and caterpillar. No part of the exoskeleton was crushed.

Gel electrophoresis lanes for CO1 arthropod PCR :

1- ladder

2- spider

3- caterpillar

4- (+) Arthropod control for arthropod test and (-) Arthropod control for wolbachia test

5- (-) Arthropod control for arthropod test and (+) Arthropod control for wolbachia test

6- DNA Control

7- water

Analysis: None of my controls worked on the wolbachia test, but my samples had no bands on the wolbachia test. I am unsure if the results are correct because the controls that had wolbachia did not have any bands on the wolbachia test and only the DNA control had a band. There could have been a problem with the electrophoresis. 

Results

Only one of the controls worked for the wolbachia test which leave me unsure if the arthropod actually had  wolbachia or not, this leaves me less confident with the results