Halyomorpha halys

November 17, 2025

Sample information
Picture
Entry by: Sahil H
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Brown body, White underside, small wings, 2 antannae, 6 legs. Quite passive in the jar.
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Isolated lower abdomen of organism by cutting with scalpel

Extracted DNA and preserved for PCR usage.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

sequenced
Wolbachia 16S sequence Download FASTA    Download AB1
GNGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGTAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATGATCAGGAATAGTAGGATCAGCCCTAAGATTAATTATCCGAATTGAATTAGGGCAACCTGGAAGATTTATTGGAGATGATCAAATCTATAATGTTGTAGTAACCGCACACGCATTTATCATAATTTTTTTCATAGTTATACCTATTATAATTGGAGGGTTTGGAAACTGATTAGTGCCTTTAATAATTGGTGCACCAGATATAGCATTCCCCCGTATAAATAACATAAGATTTTGATTATTACCACCTGCATTAACATTACTATTGATAAGAAGAATAACAGAATCAGGAGCAGGGACTGGATGAACAGTTTATCCTCCTCTATCAAGCAACATTTCACATAGTGGAGCATCTGTAGATCTAGCCATCTTTTCACTTCATTTAGCTGGAATTTCATCAATTCTAGGAGCAGTAAATTTCATTTCAACAATTATTAATATACGCCCAACAGGTATAACTCCTGAACGGACTCCCTTATTTGTCTGATCTGTAGGAGTTACAGCATTACTTTTATTACTTTCATTACCTGTATTAGCAGGGGCTATCACAATATTATTAACAGACCGAAATTTTAATACATCATTTTTTGATCCTTCAGGAGGAGGAGATCCTGTATTATATCAGCATCTATTTTGATTTTTTGGNCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halyomorpha halys was found to be postive for Wolbachia.
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