November 18, 2025

Sample information
Picture
Photos by: Zachary K.
Location
Collection date 09/30/2025
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

my arthropod was a Dark-winged Fungus Gnats its order is diptara its family is Cecidomyiidae the scientific name is Genus Afrotricha, it does have Wolbachia 
Putative identification Arthropoda Insecta Diptera Mycetophilidae

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer lysis
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

my confidence is high because i did 2 single PCR and both times mine came back positive
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Mycetophilidae was found to be postive for Wolbachia.
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