Benedictine University Student – Grace M

November 18, 2025

Sample information
Picture
Photos by: Grace M
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

found in tall grass area, in the shade
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There could have been room for human error throughout the process.
Wolbachia 16S sequence Download FASTA    Download AB1
>w14R download.ab1 (368 bp) CGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAAC TGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCC ACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCGTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAG CATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGAC AGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>1-CA_B1_CoR.ab1 (595 bp) TTAATAGCTCCTGCTAATACAGGAAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGT TTGGTCTAGTGTTATTCTTTCTGATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACAC CAGCTAAGTGAAGAGAGAAAATGGCTAAATCAATTGACGCTCCACCATGTGCAATTGCTCCAGCGAGTGGGGGGTAAACT GTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACT TATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTA TAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATT AGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATAT AAAGTATAAGAGTTTGGATTCGGGCAACCCCCGCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus was found to be negative for Wolbachia.
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