Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Founded in grass area and sidewalk |
| Putative identification | Arthropoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | There could have possibly been some room for human error |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
>89-CA_V1_CoR.ab1 (641 bp) CCTCCTCCCGCAGGGTCAAAGAATGATGTATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAATACAGG AAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTCTTTCTG ATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATG GCTAAATCAACTGACGCTCCACCATGTGCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATT ATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAG CTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAA ATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTC TGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTT T
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Acheta domesticus was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica