DEM-JCV Hemiptera

November 18, 2025

Sample information
Picture
Photos by: Jaide V.
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Greenhouse fenced in, contained tall grasses, abundance of greenery and trees
  • Environment: Hot and humid
  • Method of Collection: Sweep-Net
Putative identification Arthropoda Insecta Hemiptera Cicadellidae Homalodisca Homalodisca vitripennis

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Each control displayed the expected results, and my specimen results best matched the negative control. Moreover, the chance of my specimen having Wolbachia based on other Hemiptera collections in my area is only 1 in 4.
Wolbachia 16S sequence
Arthropod COI sequence
TTGATCTGGTATAGTTGGTATAATATTAAGAATAATTATTCGAATTGAAT TAGCTCaacctgtaggcgtttTTAATAAATGATCAATTATATAATGTAAT TGTTACTtcaCATGCTTTTATCATAATTTTTTTTATAGTTATACCAATTA TAATTGGTGGATTTGGTAATTGATTATTACCTTTAATAATTGGTGCTCCT GATATAGCATTTCCACGAATAAATAATATAAGATTTTGATTGTTACCCCC TTCATTATCTTTGTTATTAGTAAGATCAATAGTTGAAATAGGTGTTGGAA CAGGATGAACTGTTTACCCACCACTATCTTCTAATGTGGCTCATTCAGGG CCAAGAGTTGATTTAGCTATTTTTTCATTACATTTAGCAGGTATTTCATC AATTTTAGGAGCTGTAAATTTTATTACAACAGTAATTAATATACGAGCTA TTGGAATAAATTTAGATCGTACTCCTTTATTTGTTTGATCAGTATTAATT ACTGCTATTTTATTATTACTTTCATTACCTGTCTTAGCAGGTGCTATTAC TATATTATTAACTGATCGAAATATTAATACATCTTTTTTTGATCCTTCTG GTGGAGGAGATCCTATTTTATACCAACACTTATTTTGATTTTTTGGTCAC CctgaAGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Homalodisca vitripennis was found to be negative for Wolbachia.
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