Omar Alshaikh – Wolbachia Project

November 29, 2025

Sample information
Picture
Photos by: Omar A.
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Found infront of quad on the concrete right next to tall grass.
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I believe my results are correct, but there can be some slight errors in the whole process that may have given incorrect results.
Wolbachia 16S sequence
>w7R.ab1 (398 bp) AGTGTGTACAGACCCGAGACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGTACTCGAGT TGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCAT TGTAGCACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCTTATCACTGGCA GTTTCCTTAAAGTACTCAGCATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT CACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGAACCGACCCTATCCCTTCAATAGGGTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
>91-CA_V4_CoR.ab1 (650 bp) GGATCACCTCCTCCCGCAGGGTCAAAGAATGATGTATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAA TACAGGAAGTGATAATAATAAAAGGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTC TTTCTGATCGTATATTAATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAG AAAATGGCTAAATCAACTGACGCTCCACCATGTGTAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGC TCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTG GAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATA AAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCC TAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAA TATCTTTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus femurrubrum was found to be negative for Wolbachia.
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