North American Spur-throated Grasshopper

December 3, 2025

Sample information
Picture
Photos by: Zoha B
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

I used a sweep net to capture my insect which was found in a tall grass habitat in the quad.
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

In lane 4 of the gel electrophoresis, my sample did not show a DNA band and did not show a band for Wolbachia. This is why my confidence for my results are low. My controls are contradictory so I do not have confidence in my results. The W- Wolbachia did not show a DNA band and the DNA+ sample did not show any DNA band either.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Melanoplus femurrubrum was found to be negative for Wolbachia.
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