Small Milkweed Bug

December 2, 2025

Sample information
Picture
Photos by: Luis A
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 student

By Kindlon Hall using a beat net and killing jar tall grassy area
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

My wall is the fourth with little to no band, which means I did not have DNA

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

No DNA present possibly due to over dilution of my species DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
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