December 4, 2025

Sample information
Picture
Photos by: Alexa K., Sarah L., Luke H., Bransen P.
Location
Collection date 09/26/2025
Captive / Cultivated? Wild-caught
Group NCSSM
Observations

We collected this arthropod by using a small net and jar to capture the bugs that were attracted to outside lights.
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We had strong lines in our gel electrophoresis and we had consistent Wolbachia results throughout.
Wolbachia 16S sequence
Arthropod COI sequence
TAGGAACATCCCTCAGACTATTAATTCGAGCAGAATTAGGTACCCCTGGATCTTTAATTGGAGATGATCA AATTTATAATACTATTGTTACAGCACATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATT GGAGGATTTGGTAACTGATTAGTACCTCTTATATTAGGGGCCCCTGATATAGCATTTCCCCGAATAAATA ATATAAGTTTTTGATTATTACCCCCTTCTTTAACTCTTCTAATTTCAAGAAGTATCGTAGAAAATGGAGC AGGAACTGGGTGAACAGTTTATCCACCTTTATCATCTAATATTGCTCATGGGGGGAGATCAGTAGATTTA GCTATTTTCTCTCTTCATTTAGCTGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTACTACAATTA TTAATATACGTCTTAATGGATTATCCTTTGATCAAATACCTTTATTTATTTGAGCTGTAGGAATTACAGC TTTTTTACTTCTTTTATCCCTACCTGTATTAGCTGGAGCAATTACAATATTATTAACTGATCGAAATCTT AATACTTCTTTTTTCGATCCTGCTGGAGGAGGAGAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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