Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/25/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | NCSSM |
| Observations | The hill we needed to break had many clusters worth of eggs in it.
|
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Solenopsis Solenopsis invicta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | eggs |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | Cyber Green |
| Gel images |
|
| Protocol notes | Since these were eggs, we used two or three instead of just one to be positive we got some workable DNA. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The arthropod that comes from these eggs is known to have Wolbachia in other parts of the world (central and south America) |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Solenopsis invicta was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica