Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students using sweep net, found in tall grass |
| Putative identification | Arthropoda Insecta Blattodea Ectobiidae Blattella Blattella germanica |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Due to human error I was unable to produce a successful DNA strand to go into the gel electrophoresis. After the class data, it was extremely light/faint and I was unable to see my entire column correctly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Blattella germanica was found to be negative for Wolbachia. |
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica
Armadillidium nasatum