Armadillidium nasatum

December 4, 2025

Sample information
Picture
Photos by: Zayna M.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by fall 2025 students, found under a rock near the lake.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium nasatum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My band was very faint
Wolbachia 16S sequence Download FASTA   
GCAAGTCCCAACTAAGGTGCGCGGGACTTAACCCAACATCTCACGACACGACGCCATGCACACTTGTGTAAAATCCGGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
GTTTAAATTACGGCAGTCAAATAGCTCCCAACAGGAAAGATAAGAGAAAAAACCGCGGTTACTAAAACAGACCAAACAAA TAAAGGACCGATCCATACTAATTCCGGACGCCCGATATTAATTATAGGAATAAAGTTTACTGCCCCATTGATGAAGCCCC AAAGATGAAAGAAAAAACCTAAATCTACAGAAGCTCCACTATGGGCAATTCTTGATGCCAAAGGAGGGAAACAGTTCATC CCGTTCCACTCCTCTTTCAATTAATCCTCTGCTAAGGAGTAAAGTTAAAGAAGGGGGAAGCAGCCAAAATCTTATATTGT TTATAGAAAGCTATATCAGGAGCCCCTAATATCAAGGAATTAATCAATTTCCAAACCTCCATTATAATAGGATTACTATG AAAAAATCATTACAAAAGCATGACGGACATTACATTATAAGATCATCCAATGAGACTCCCAGTGCCCTAATTCCCGAATA ATTATTCTTAAAGGGACCACACTGCCAAACTCCGATATAAAATATAATGTACCAATATTTTATGAGTGCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium nasatum was found to be negative for Wolbachia.
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