Sample information
Picture	Photos by: Jana S
Location
Collection date	12/04/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	collected by Fall 2025 students
Putative identification	Arthropoda Insecta Diptera Tipulidae Nephrotoma
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	my percent identity was 99.48%.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Nephrotoma was found to be postive for Wolbachia.