Wolbachia data

December 4, 2025

Sample information
Picture
Photos by: Hafsah M
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

It has

Head: 

  • Antennas 
  • Mouthparts 

Thorax: 

  • Seven segments

Abdomen:

  • Six segments
  • Breathing gills

scaly back, mouth parts, 14 legs
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I am not as confident because I properly followed the Wolbachia project lab protocols and completed the instructions but there was error finding wolbachia which could be due to salt contamination or the PCR conditions failed to find Wolbachia DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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