Sample information
Picture	Photos by: Brissa A
Location
Collection date	09/03/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by fall 2025 students
Putative identification	Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Green
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Results were not accurate since it was a different species
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Melanoplus femurrubrum was found to be negative for Wolbachia.