Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2025 students Caught by Maple Tree nearby the lake |
| Putative identification | Arthropoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The blast match result was Forficula with the following data: Query Coverage: 100% E value: 0.0 % identity: 100.00% |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Forficula auricularia was found to be negative for Wolbachia. |
Grasshopper, student Wolbachia project
Wolbachia Project (Bombus Terrestris)
Vespula
Bombus Affinus (Rusty Patch Bumblebee)
European Earwig