Bombus Affinus (Rusty Patch Bumblebee)

December 5, 2025

Sample information
Picture
Photos by: Sidrah A.
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students. Caught using a sweep net and a kill jar.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Wolbachia was not present. Some mistakes were made when micropipetting controls during gel electrophoresis. Blast and phylogenetic results are not reliable as they did not match anatomy of arthropod or order of arthropod.

Blast results as follows:

Bascillus sp.

Query: 20%

E-Value: 1e-21

Percent Identity: 85.34%
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>60-AA-Y1_CoR.ab1 (956 bp) AGCCCGGTGCGCGGAGAACGGTTGTTTCCAAAGGTAACCTCCCAATTTCGGGGGTTACAAACTCTCGGGG GGTGACTGGGGGGGGGTACAGGGCCCGGGAGATATTCACCGCGAATTGGTGATGCTCGATTATTAGAGAC TCCTACTACTTGAGGGAGAGGGGTAGCCGACAACGCACAATGATAAAAATTTTTTGAAAAAATTTCCTTC CCCCCGAGTCCCCGCTCCTTGTGGCACCCTGTGTGGCAGGTGTGGAGCCCGCCACGTAAGGAGCGGGATG ATGTGATGGTTCCTCGTTTCTTCCGGGGGGTTACCGGGGGGTTCTGTAAAGCCGCCCCTATATGATGGTT ACTAACAATAAAGGGGGGGGTCCTTCGGGGTTTACTTAACCAACTCCCTACCAAAACTAGCGACCAACAT CCTCCCCCTTGTTTCAATTTCCCGAAAGGGCTAACTTTTTTTTGAAATAGTCTCAGGATGTCAAGACCTG GTAAGGTTCTTCCCTTTACTTGAATTGAACCACATGCTCTACGCCGTGTGGGGGCCCCCCCCTTCTCTTT TTTTTTGATTTGGGCCCGGGGTCCCCCCCCCCAGAATTATATATTATATTTTTTGCTGCACGCTCAAAGG AACCAACCCCCCACCAACTACTGTTCATTTTGGGGGGCAGGAGCACCAAATCTTGTTGTTTCCTCTGCTC TCCCTCTCAGCCCAGCTCAGATACAGAGAAAAAAGACACATTCGCCATCGGCGGACTCCTCGTATCTACG CCTTTACAACAGGACACATGAGAATTCTCCTCTCCTCTCCCGTCATGCAAGTTAGAAGGGTTCTACAAGA AAGACCGGGCAATGTCAAGTTTAATAAAAATAAACGTATAAAGACGCTCGGGCCCCGTTACTCCCAAAAA AATCAACCCCCATATTAGGGGAGGGTTGGGGAAGGGTGCGGGGATG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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