silk-spinning cricket

December 7, 2025

Sample information
Picture
Photos by: Lontrez D.
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations
  • Collected Fall 2025
  • Sweep net
  • Found by the pond closest to our lab building (BK)
  • I apprehended the insect while it was hopping mid-air through the grass and dirt
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Limiting as much debrief and contamination as we can during DNA extraction and purification.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

I would be much more confident of my results if our gel electrophoresis lab were successful. And this may be due to human or technical error playing a big part in seeing if the insects contained Wolbachia in the DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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