Sample information
Picture	Photos by: Michaela A.
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by fall 2025 students
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.