Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | The Roly-Poly was under a rock near the bushes. It was picked up with tweezers and it curled up when trying to pick it up to put in a container. |
| Putative identification | Arthropoda Malacostraca Isopoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
GACCATTAAAGGATAAGGGGTCCCCCCCTCTCCGGGGGGATTATATAAATTAATTTTTATGTGATTAAAAATTGGCTTGG CGTGGAACAGGGAGAGAGATAAAATAAAAGATTGTTGTTACTACTGACCTACACTAAGGGTATTCGATTAAGTGAATTTC GTGGGGTGCGTATATTAATGACGGAGAAATAAAATTAACTACTGCTAAAATTGAAGAAGTGCCTGCTGGATGATGAGAAA AAATAGTTACATCTTCTGATGCCCCCCCATGAGCAATTACTGAAGAAAAAGGAGGATGATCAGCTCCTCCTGTTCCTCCT CCTTTTATACTATACTTGTTGCTAATATAAAAGATGGAGGAGGAGGTAAAACTCAAAATCTAATATTATTTATTTAAGCA AATGTTATATCTATGAGCACATATTAATAAAGGAACTAATTAATCCCCAAACCTACCAATTATTTTTGATATAACTTTAT AAAAATTTATAAGAAAAGAATGAGTGGGCTCAATTACTTTATTTATTTGATTATCCCCAACCAATGCTCCTGGATGTACT ACTTCAGTTCGAATTAATTAATATATAGATGTTTATACTATTTCAGCCCACGCTTATTACAATAAAATATAAAGTTTCTT ATTTTGTTTACC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Isopoda was found to be negative for Wolbachia. |
Gryllus sp. (Cricket)
Sciara hemerobioides
Polistes dominula (European paper wasp) – Andriana Vishka
(no title)
Mosquito – Anopheles gambiae