Harmonia axyridis

December 8, 2025

Sample information
Picture
Photos by: Maria Botros
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

The insect was collected within the Benedictine university’s area. It specifically was located near a lake that is close to the brick building. It was collected with a tweezer and container. Apparently, it was found on one of the large tree’s leaves near the brick lake.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

My confidence level is low since my precent identification is below 99%. In addition, the gel electrophoresis band was little faded.
Wolbachia 16S sequence Download FASTA    Download AB1
>67-FW_B2_WoR.ab1 (394 bp) CCACTTTGGATGTGAGAGTGGTGCCTCGTCGCTGTGGCTGTTTACATTATATTAGTGATTCCAATAGGAGGTAGAGGTGC AGAGAACACTCGAACTGAGATGGTTTTTATTGGATTATGTTAGCCTCTGAACTTTGCAGCCCATTGTACCCACCATTGTA GCCCGAGGGAAGCCCACTCCATACGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTGATCAGTGGGATTTT CCTTAAATCCCCCATCATTACCTGATGGTAATTAAGGATGAGGGTTGCGCTCGTTGGGGGACTTAACCCACCATCTCACG ACACGTTCTGACGACAGCCATGTGACACCTGGGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATATGTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>39-FA_B2_CoR.ab1 (494 bp) GCCCCCCGGTGAAAAGATTTAAGTTTCGGTTAATATATAGTAATTGCTCCTGCAAGAACTGGAGATTAAAAGAATTGTGT AATAAGAACAGATCATACAAATAAAGGAGTTTTATCAAGATTATACCAAATGGACGTATATTTATAATATTGAAATGAAA TTTACTGCACCTAAAAGATTCGTGTTACGCCAGAATAACAGTCATCCTGTTCCTGCCCCTATTTCTACATTGTTCTTAAA ATTAAAAGAGTTAAAGCAGGGGGAAAGTCAAATCTTATGTTATTTAATCGGGAAATGCTATATCAGACTCCATTATTAAA GGAACTACATTTCCAAACCCCCAATTATAATAGTATTACTATAAAGAAAATTATAAGAATGACTGAACAATTATATTATA ATTTGGCGTTCCAATTACCTCTATCCTAATTCTAACAATTAATACTAACGATGTTCCACTATTCCTGCCCATATTCCAAA TAAAAATATAATGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be postive for Wolbachia.
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