Sample information
Picture	Photos by: Journey G
Location
Collection date	09/02/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected off of tree near a lake
Putative identification	Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit.
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA
Summary	The Harmonia axyridis was found to be negative for Wolbachia.