Pseudolycoriella sp. (Black fungus gnat)

December 8, 2025

Sample information
Picture
Photos by: Sarah S
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

This arthropod specimen is a black fungus gnat that belongs to the genus Pseudolycoriella Sp. It was collected outdoors at Benedictine University and is a small, dark colored fly commonly found in moist environments. The fungus gnat has thin long legs, one pair of wings, and a slender body, which are all characteristic features of dipteras.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident in these results since both the positive and negative controls worked the way they were supposed to. The positive control showed a clear Wolbachia band at the expected size, which confirms that the PCR worked correctly. The negative control showed no bands, showing that no contamination was present. My arthropod sample showed a strong COI band, proving that the DNA extraction and insect DNA amplification were successful, but no Wolbachia band was present. These results support the conclusion that my fungus gnat specimen was not infected with Wolbachia.
Wolbachia 16S sequence Download FASTA   
>60-DW_B4_WoR.ab1 (305 bp) TGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAACCCATTGTAGACACCAT TGTAGCACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCTTATCACTGGCA GTTTCCTTAAAGTACTCAGCATTACCTGATAGCAACTAAGGATGAGGGTTGCACTCGTTGCGGGACTTAACCCAACATCT CACGACACGAGCTGACGACAGCCATGCAACACTTGTGTGAAATCCGGCCGAACCGACCCTATCCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
>13-DA_G1_CoR.ab1 (524 bp) CAGTAATAAATACGGATCACACAAATAAAGGTATTTTATCAAATGTTATTCCTGGGGCTCGTATATTAATAATTGTTGAA ATAAAATTTACAGCCCCTAAAATTGATGAAATACCTGCAAGATGTAAGGAAAAGATTGAAAGATCTACAGAGGCCCCCGT ATGTGCAATAGTTGAAGACAAAGGAGGATAAACTGTTCAACCTGTTCCAGTTCCTCTATCTACCAAACTACTTGTTAATA AAAGAGTTAATGATGGAGGTAATAACCAAAATCTTATATTATTTAATCGGGGAAATGCTATGTCAGGGGCGGATAATATT AATGGAACTAATCAATTTCCAAATCCTCCAATTATAATTGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCAGT TACAATTACATTATAAATTTGATCATCTCCAATTAAAGCAAATGGATAACCTAATTCTGCTCGAATTAATATACTTAATG ATGTTCCTACTATTCTAGACCACGCCCCAAAAATAAAATATAAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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