American grasshopper

December 8, 2025

Sample information
Picture
Photos by: Hilarie S
Location
Collection date 12/08/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students.
Putative identification Arthropoda Insecta Diptera Culicidae Anopheles

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

My numbers were within the minimum numbers.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My insect was Wolbachia positive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Anopheles was found to be postive for Wolbachia.
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