|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
antennas, 6 legs, and black
|Putative identification||Hexapoda Insecta Hymenoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
Not enough supernatant and pipetted tiny tubes wrong. The pellet was small but visible.
|Explanation of confidence level||
DNA ladder in arthropod gel was not properly loaded. Positive and negative controls did not work. Wolbachia gel control did not work, may be problems with the DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hymenoptera was found to be negative for Wolbachia.|