Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/27/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | antennas, 6 legs, and black |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Not enough supernatant and pipetted tiny tubes wrong. The pellet was small but visible. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | DNA ladder in arthropod gel was not properly loaded. Positive and negative controls did not work. Wolbachia gel control did not work, may be problems with the DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman