|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
The Arthropod has 3 sections. It has six legs. It also has a pair of wings; the wings are split into two sections. It also has hair all around its body including the head. It has a long nose. It also has red eyes which occupy the majority of the head.
|Putative identification||Hexapoda Insecta Diptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
The pellet is translucent around perimeter
|Explanation of confidence level||
DNA ladder in arthropod gel was not properly loaded; positive and negative controls did not work. Wolbachia gel control did not work; maybe problems with DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Diptera was found to be negative for Wolbachia.|