Sample information

Very small insect with wings, 4 limbs, tan/orange body, dark face.

Methods

Place the gel tray containing your gel in the buffer chamber
Ensure that the clear buffer chamber is inside the blueGel electrophoresis system.

The wells of the gel should be on the same side as the negative electrode, away from the power button.

Use a graduated cylinder to add 30 ml of 1X TBE electrophoresis buffer
The buffer should just cover the gel and wells.

Ensure that there are no air bubbles in the wells (shake the gel gently if bubbles need to be dislodged).

Starting with the ladder in lane 1, gently hover your pipette tip over the well, and load 5 uL. Follow the loading key in the table below to load the rest of your gel. Remember to change tips between each sample.

Results

The majority of the lab was carried out by my peers in my absence so I don’t have a strong grasp on the materiel & results.