8N

January 26, 2026

Sample information
Picture
Location
Collection date 09/07/2025
Captive / Cultivated? Captive / Cultivated
Group St. Paul Academy and Summit School
Observations

I was given this cricket by my sister because she had a lot of them for feeding her pet leopard gecko. They were purchased from Petco. It was kept at room temperature in the store. I do not know where Petco gets all of these crickets.
Putative identification Arthropoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was a very clear line confirming that it was, in fact, an arthropod, but there was no second line.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Acheta domesticus was found to be negative for Wolbachia.
Report Inappropriate Post