Sample information
Picture
Location
Collection date	09/08/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Collected on tennis courts, near a grassy area, in temperatures around 75 degrees Fahrenheit.
Putative identification	Arthropoda Insecta Hymenoptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	agarose gel electrophoresis
Buffer
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be postive for Wolbachia.