7A

February 17, 2026

Sample information
Picture
Entry by: Kieran A.
Location
Collection date 09/06/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found this leaf bug in my yard. It landed on my sister’s backpack, and I collected it there. It was around noon that I collected it. It was sunny outside.
Putative identification Arthropoda Insecta Hemiptera Acanaloniidae Acanalonia

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TBE
DNA stain SeaGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked as expected, and although we did not run a ladder in this specific gel, our class’ data was split into two gels, and the other group ran a ladder, and it worked as expected. All of our arthropod DNA bands in the gel were exactly at the same length. Also, my arthropod DNA was in lane 2 (7A).
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Acanalonia was found to be negative for Wolbachia.
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